Multienzymatic Assemblies: Methods and Protocols

1. Recombinant Expression and Purification of Large Bacterial Multienzyme Assemblies for Biosynthetic Processes

James Christenson

2. Multi-Dimensional Fluorescence Live-Cell Imaging for Glucosome Dynamics in Living Human Cells

Songon An, Prakash Parajuli, Erin L. Kennedy, and Minjoung Kyoung

3. Mechanisms and Effects of Substrate Channelling in Enzymatic Cascades

Svyatoslav Kondrat and Eric Von Lieres

4. Structural Characterization of Multienzyme Assemblies: An Overview

Anastassios C. Papageorgiou

5. Assessing Protein Interactions for Clustering of Mitochondrial Urea Cycle Enzymes

Ljubica Caldovic, Shivaprasad Bhuvanendran, and Jyoti Jaiswal.

6. DNA Nano-Scaffolds for Multienzyme Systems Assembly

Sung Won Oh, Zhicheng Wang, and Jinglin Fu

7. Strategies for Multienzyme Assemblies

Qing Sun, Maryam Raeeszadeh-Sarmazdeh, Shen-Long Tsai, and Wilfred Chen

8. Random and Positional Immobilization of Multi-Enzyme Systems

Hassan Maleki, Kamyar Khoshnevisan, and Hadi Baharifar

9. Compartmentalized Immobilization of Multi-Enzyme Systems

Hadi Baharifar, Kamyar Khoshnevisan, and Hassan Maleki

10. 3D Printed Polylactic Acid Well-Plate for Multi-Enzyme Immobilization

Elena Gkantzou, Anastasia Skonta, Andreas-Georgios Vasios, Haralambos Stamatis

11. Rational Design of Self-Assembling Supramolecular Protein Nanostructures Utilizing The Cucurbit[8]Uril Macrocyclic Host

Elisavet Ioannou and Nikolaos E. Labrou

12. Lectin-Mediated Coimmobilization of Cascade Glycoenzymes

Yifei Zhang, Yunzhenshan Gao, and You Yong

13. Self-Assembled Multienzyme Nanostructures for Biocatalysis In Cellulo

Qixin Wei and Jiang Xia

14. Nanoarmored Multi-Enzyme Cascade Catalysis

Mansi Malhotra, Ankarao Kalluri, and Challa Vijaya Kumar

15. Implementing Multi-Enzyme Biocatalytic Systems Using Nanoparticle Scaffolds

Joyce C. Breger, Gregory A. Ellis, Scott A. Walper, Kimihiro Susumu, and Igor L. Medintz

16. A Four-Enzyme Nanoassembly Consisting of Hydrolases and Oxidoreductases for Multi-Step Cascade Reactions

Archontoula Giannakopoulou, Michaela Patila, Nikolaos Chalmpes, Dimitrios Gournis, and Haralambos Stamatis

17. A Bi-Enzymatic Immobilized Nanobiocatalyst for The Biotransformation of Oleuropein to Hydroxytyrosol

Archontoula Giannakopoulou, Alexandra V. Chatzikonstantinou, Aliki Tsakni, Christos L. Chochos, Dimitra Houhoula, and Haralambos Stamatis

18. A Simple Recombinant E. coli Cell Lysate-Based Biocatalyst for ATP-Dependent Multi-Step Reactions

Natalia Bartzoka, Thomas D. Loan, Hideki Onagi, and Apostolos Alissandratos

19. CO2 to Methanol: A Highly Efficient Enzyme Cascade

Io Antonopoulou, Ulrika Rova, and Paul Christakopoulos

20. Multienzyme Catalysis in Phase-Separated Protein Condensates

Miao Liu, Xi Chen and Jiang Xia

21. Robust and Continuous Lipase-Catalyzed Reactions in Deep Eutectic Solvents: Low Viscosity and Double CLEA-Lentikats Immobilization (CLEA-LK-Lipases)

Nadia Guajardoa and Pablo Domnguez De Mara

22. Enzymatic Photometric Assays for the Selective Detection of Halides