Gene Therapy of Cancer: Methods and Protocols

1. Brief Introduction to Current Cancer Gene Therapy

Dennis Kobelt, Jessica Pahle, and Wolfgang Walther

Part I: Strategies in Cancer Gene Therapy

2. Minicircles for CAR T Cell Production by Sleeping Beauty Transposition - A Technological Overview

Sabrina Prommersberger, Razieh Monjezi, Ram Shankar, Marco Schmeer, Michael Hudecek, Zoltn Ivics, and Martin Schleef

3. Generation of CAR-T Cells with Sleeping Beauty Transposon Gene Transfer

Sabrina Prommersberger, Razieh Monjezi, Lacramioara Botezatu, Csaba Miskey, Maximilian Amberger, Katrin Mestermann, Michael Hudecek, and Zoltn Ivics

4. Generation of Redirected Engineered Human Chimeric Antigen Receptor (CAR) T cells

Mario Bunse and Uta E. Hpken

5. TCR- and CAR-engineering of Primary Human T cells

Inan Edes, Julian Clauss, Rainer Stahn, Alberto Sada Japp, and Felix K. M. Lorenz

6. TCR Gene Therapy for Cancer

Simone Rhein and Neş
e akmak-Grr

7. Gene Gun Her2/neu DNA Vaccination: Evaluation of Vaccine Efficacy in a Syngeneic Her2/neu Mouse Tumor Model

T. Nguyen-Hoai, O. Hohn, A. Pezzutto, and J. Westermann

Part II: Suicide Gene Therapy

8. Construction of Minicircle Suicide Genes Coding for Ribosome-Inactivating Proteins

Alexander Sonntag, Hardy Mitdank, and Alexander Weng

9. Claudin-targeted Suicide Gene Therapy for Claudin-Overexpressing Tumor Cells by Using Modified Clostridium Perfringens Enterotoxin (CPE)

Laura-Sophie Beier, Jrg Piontek, Anna Piontek, Jonas Protze, Dennis Kobelt, and Wolfgang Walther

Part III: Gene Suppression and Signaling Modulation Therapy for Cancer

10. Bioengineered RNA Therapy in Patient-Derived Organoids and Xenograft Mouse Models

Mei-Juan Tu, Colleen M. Yi, Gavin M. Traber, and Ai-Ming Yu

11. Application of Transcription Factor Decoy Oligodeoxynucleotides (ODNs) for Cancer Therapy

Behrooz Johari and Mohammad Moradi

Part IV: Oncolytic Therapy for Cancer

12. NK Cell Effector Functions and Bystander Tumor Cell Killing in Immunovirotherapy Alessia Floerchinger and Christine E. Engeland

13. A qPCR-based Method for Quantification of Replication Competent Adenovirus (RCA) in Conditionally Replicating Oncolytic Adenoviruses

Erik Yngve, Menghan Gao, and Di Yu

14. Generation of a microRNA-regulated oncolytic Coxsackievirus B3

Babette Dieringer, Leslie Elsner, Ahmet Hazini, Jens Kurreck, and Henry Fechner

15. Visualized Cancer Immunotherapy with Engineered Salmonella typhimurium

Yujie Sun and Jin Hai Zheng

Part V: Translational Aspects for Cancer Gene Therapy

16. Cancer Gene Therapy: Development and Production of Lentiviral Vectors For Gene Therapy

Ana S. Coroadinha

17. Capillary Gel Electrophoresis (CGE) for Quality Control of Plasmid DNA in Gene Therapy: quality control of 20-years stored GMP-grade Plasmid DNA

Schmeer M, Schleef M, Shankar R, Kobelt D, and Walther W

18. Efficient Generation of Superior Dumbbell-Shaped Non-viral DNA Delivery Vectors Using 1-2-3 Gap-primer PCR

Pei She Loh and Volker Patzel