Oral Biology: Molecular Techniques and Applications

Part I: Saliva and Other Oral Fluids

1. RNA Sequencing Analysis of Saliva exRNA

Karolina Elż
bieta Kaczor-Urbanowicz and David T.W. Wong

2. Proteome Analysis of Oral Biofluids in Periodontal Health and Disease Using Mass Spectrometry

Nagihan Bostanci and Kai Bao

Pingping Han, Xiang Li, Wei Wei, and Saso Ivanovski

4. Antioxidant Micronutrients and Oxidative Stress Biomarkers

Irundika H.K. Dias, Helen R. Griffiths, Mike R. Milward, Martin R. Ling, Iain L.C. Chapple, and Melissa M. Grant

Part II: Molecular Biosciences

5. The Oral Microbiota in Health and Disease: An Overview of Molecular Findings

Jos F. Siqueira Jr and Isabela N. Ras

6. The Long and Short of Genome Sequencing: Using a Hybrid Sequencing Strategy to Sequence Oral Microbial Genomes

Nicholas C.K. Heng and Jo-Ann L. Stanton

7. Microbial Community Profiling Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) and Denaturing Gradient Gel Electrophoresis (DGGE)

Jos F. Siqueira Jr, Mitsuo Sakamoto, and Alexandre S. Rosado

8. Bioinformatic Approaches for Describing the Oral Microbiota

Kristi Biswas, Michael W. Taylor, and David T.J. Broderick

9. Adhesion of Yeast and Bacteria to Oral Surfaces

Richard D. Cannon, Karl M. Lyons, Kenneth Chong, Kathryn Newsham-West, Kyoko Niimi, and Ann R. Holmes

10. Quantitative Analysis of Periodontal Pathogens Using Real Time Polymerase Chain Reaction (PCR)

Ma Jos Marin, Elena Figuero, David Herrera, and Mariano Sanz

11. Methods to Study Antagonistic Activities Among Oral Bacteria

Fengxia Qi and Jens Kreth

12. Generation of Multi-Species Oral Bacteria Biofilm Models

Jason L. Brown, Mark C. Butcher, Chandra Lekha Ramalingam Veena, Safa Chogule, William Johnston, and Gordon Ramage

13. Markerless Genome Editing in Competent Streptococci

Roger Junges, Rabia Khan, Yanina Tovpeko, Heidi A. mdal, Fernanda C. Petersen, and Donald A. Morrison

14. A Protocol to Produce Genetically Edited Primary Oral Keratinocytes Using the CRISPR-Cas9 System

Sven E. Niklander and Keith D. Hunter

15. Size-Based Method for Enrichment of Circulating Tumor Cells from Blood of Colorectal Cancer Patients

Sai Shyam Vasantharajan, Edward Barnett, Elin S. Gray, Euan J. Rodger, Michael R. Eccles, Sharon Pattison, Fran Munro, and Aniruddha Chatterjee