2-D Proteome Analysis Protocols

Name: 2-D Proteome Analysis Protocols
Brand: Humana Press Inc.
SKU: 9781617370601
Price: 63062 HUF
Availability: InStock

Szerző: Andrew J. Link

Nyelv:

Kötés: Puha kötésű

Kiadó: Humana Press Inc.

Elérhetőség: Beszállítói készleten alacsony példányszámban

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With the completion of sequencing projects and the advancement of a- lytical tools for protein ident...

Információk a könyvről

Szerző

Andrew J. Link

Nyelv

Angol

Kötés

Könyv - Puha kötésű

Kiadva

2010

oldal

601

EAN

9781617370601

ISBN

1617370606

Enbook ID

02717249

Kiadó

Humana Press Inc.

Súly

899

Méretek

152 x 229 x 35

Teljes leírás

With the completion of sequencing projects and the advancement of a- lytical tools for protein identification, proteomics the study of the expressed part of the genome has become a major region of the burgeoning field of functional genomics. High-resolution 2-D gels can reveal virtually all p- teins present in a cell or tissue at any given time, including posttranslationally modified proteins. Changes in the expression and structure of most cellular proteins caused by differentiation or external stimuli can be displayed and eventually identified using 2-D protein gels. 2-D Proteome Analysis Protocols covers all aspects of the use of 2-D protein electrophoresis for the analysis of biological problems. The contri- tors include many of the leaders in the fields of biochemistry and analytical chemistry who were instrumental in the development of high-resolution 2-D gels, immobilized pH gradients, computer analysis, and mass spectromet- based protein identification methodologies. This book is intended as a benchtop manual and guide both for novices to 2-D gels and for those aficionados who wish to try the newer techniques. Any group using protein biochemistry especially in the fields of molecular biology, biochemistry, microbiology, and cell biology should find this book eminently useful. 2-D Proteome Analysis Protocols takes the researcher through the c- plete process of working with 2-D protein gels from making the protein - tract to finally identifying the proteins of interest. It includes protocols for generating 2-D protein extracts from most of the standard model organisms, including bacteria, yeast, nematode, Drosophila, plants, mouse, and human.

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2-D Proteome Analysis Protocols

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