A Green Fluorescent Protein-Based Purification System for Plant Cells

Name: A Green Fluorescent Protein-Based Purification System for Plant Cells
Brand: VDM Verlag Dr. Müller
SKU: 9783639126426
Price: 21137 HUF
Availability: InStock

Optimizing Yield and Recovery of Recombinant Proteins from Plants

Szerző: Gabriel Peckham

Nyelv:

Kötés: Puha kötésű

Kiadó: VDM Verlag Dr. Müller

Elérhetőség: Kiadói készleten rendelésre

Küldés 17-27 napon belül

21 137 Ft

Purifying recombinant proteins from plants is often met with difficulties due to the complexity of p...

Információk a könyvről

Szerző

Gabriel Peckham

Nyelv

Angol

Kötés

Könyv - Puha kötésű

Kiadva

2009

oldal

148

EAN

9783639126426

Enbook ID

06821417

Kiadó

VDM Verlag Dr. Müller

Súly

237

Méretek

150 x 220 x 9

Teljes leírás

Purifying recombinant proteins from plants is often met with difficulties due to the complexity of plant cell extracts. Issues include high levels of protease activity, tannin and phenolic reactivity and hydrophobic aggregation. Difficulties in extraction and purification are major hurdles preventing the routine use of plants and plant suspension cells as bioreactors. Reporter molecules, such as green fluorescent protein, are often used to monitor recombinant protein expression levels. Therefore, a purification strategy was developed taking advantage of this commonly used reporter molecule. Although previous methods have been developed for purifying GFP, these strategies often included exposure to protein-denaturing solvents or required gel filtration and protein concentrating steps. This work details a strategy that optimizes yield and purity without jeopardizing the integrity of the fusion protein.

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A Green Fluorescent Protein-Based Purification System for Plant Cells

Optimizing Yield and Recovery of Recombinant Proteins from Plants

Információk a könyvről

Teljes leírás

Érdekelheti

Azok a vásárlók, akik ezt a könyvet megvásárolták, a következőket is megvásárolták