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Könyv Bacterial Protein Secretion Systems Laure Journet

Bacterial Protein Secretion Systems

Szerző: Laure Journet, Eric Cascales
Nyelv: Angol
Kötés: Kemény kötésű
Kiadó: Humana Press Inc.
Elérhetőség: Beszállítói készleten
Küldés 10-13 napon belül
86 651 Ft
This volume details protocols that cover the broad arsenal of techniques used to study a secretion s...

Információk a könyvről

Szerző
Laure Journet, Eric Cascales
Nyelv
Angol
Kötés
Könyv - Kemény kötésű
Kiadva
2017
oldal
528
EAN
9781493970315
ISBN
1493970313
Enbook ID
15975773
Kiadó
Humana Press Inc.
Súly
1216
Méretek
178 x 254 x 35

Teljes leírás

This volume details protocols that cover the broad arsenal of techniques used to study a secretion system from A to Z. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as reporters to follow effector transport. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Protein Secretion Systems: Methods and Protocol aims to provide techniques that are not restricted to the study of secretion systems but are also of specific interest for any researcher interested on multi-protein complexes of the bacterial cell envelope.

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