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Könyv Cell-Free Gene Expression Ashty S. Karim

Cell-Free Gene Expression

Methods and Protocols

Szerző: Ashty S. Karim, Michael C. Jewett
Nyelv: Angol
Kötés: Puha kötésű
Kiadó: Springer, Berlin
Elérhetőség: Beszállítói készleten
Küldés 10-18 napon belül
54 382 Ft
This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable ne...

Információk a könyvről

Szerző
Ashty S. Karim, Michael C. Jewett
Nyelv
Angol
Kötés
Könyv - Puha kötésű
Kiadva
2023
oldal
435
EAN
9781071620007
Enbook ID
42644638
Kiadó
Springer, Berlin
Súly
784
Méretek
178 x 254

Teljes leírás

This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling high-throughput cell-free experimentation. The second section provides an array of applications for CFE systems, such as metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cell-Free Gene Expression: Methods and Protocols serves as an ideal guide for researchers seeking technical methods to current aspects of CFE and related applications.

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