Clinical Applications of Capillary Electrophoresis

Name: Clinical Applications of Capillary Electrophoresis
Brand: Humana Press Inc.
SKU: 9781489941749
Price: 38736 HUF
Availability: InStock

Szerző: Stephen M. Palfrey

Nyelv:

Kötés: Puha kötésű

Kiadó: Humana Press Inc.

Elérhetőség: Beszállítói készleten

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The term electrophoresis was first used by Michaelis in 1909, to - scribe the migration of colloids...

Információk a könyvről

Szerző

Stephen M. Palfrey

Nyelv

Angol

Kötés

Könyv - Puha kötésű

Kiadva

2013

oldal

212

EAN

9781489941749

ISBN

1489941746

Enbook ID

05182162

Kiadó

Humana Press Inc.

Súly

340

Méretek

152 x 229 x 13

Teljes leírás

The term electrophoresis was first used by Michaelis in 1909, to - scribe the migration of colloids in an electric field. The first practical elect- phoresis method was described by Tiselius in 1937. He used a U-tube filled with buffer layered on top of sample; migration could be monitored using Schlieren optics. In zone electrophoresis, the U-tube was replaced by paper, a support material employed simply to prevent or minimize diffusion of ions, so that ions applied in a narrow strip to the paper will separate and remain as relatively discrete zones. Paper was superceded by a variety of other media, - cluding cellulose acetate, hydrolyzed starch (starch gel), agarose, and polyacry- mide. The latter, in addition to being a support medium, has size-sieving properties. From the basic zone electrophoresis, other means of separation have been dev- oped. These include, isoelectric focusing, isotachophoresis, density gradient el- trophoresis, and various forms of immunoelectrophoresis. In some ways Capillary Electrophoresis (CE) has gone full circle back to the original method of Tiselius. In its simplest form, separations occur in a buffer solution within a glass (fused silica) tube and detection occurs as sample moves past an optical window. CE has rapidly developed into a technique that rivals HPLC in its versatility. All the classical electrophoretic separations zone, IEF, and isotachophoresis have their counterparts in CE. Excitingly so, and - thoritatively treated in Clinical Applications of Capillary Electrophoresis.

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Clinical Applications of Capillary Electrophoresis

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