Heterologous Gene Expression in E.coli

Name: Heterologous Gene Expression in E.coli
Brand: Humana Press Inc.
SKU: 9781617379666
Price: 57610 HUF
Availability: InStock

Methods and Protocols

Szerző: Thomas C. Evans, Ming-Qun Xu

Nyelv:

Kötés: Kemény kötésű

Kiadó: Humana Press Inc.

Elérhetőség: Beszállítói készleten

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Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotech...

Információk a könyvről

Szerző

Thomas C. Evans, Ming-Qun Xu

Nyelv

Angol

Kötés

Könyv - Kemény kötésű

Kiadva

2010

oldal

310

EAN

9781617379666

ISBN

1617379662

Enbook ID

01432517

Kiadó

Humana Press Inc.

Súly

764

Méretek

187 x 264 x 22

Teljes leírás

Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. Despite the advanced state of protein expression technology improvements are still needed. For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host. The ideal host would have the ability to express any protein, with relevant post-translational modifications, and be as easy to work with as E. coli. In Heterologous Gene Expression in E. coli: Methods and Protocols, expert scientists intimately familiar with the relevant techniques offer chapters that greatly expand the utility of this expression host. The contributions in this detailed volume describe methods, for example, to successfully express proteins in E. coli that would otherwise form aggregates in this host, to add post-translational modifications, to incorporate non-standard amino acid residues or moieties into E. coli expressed proteins, to identify binding partners, and to express membrane proteins. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.§Practical and cutting-edge, Heterologous Gene Expression in E. coli: Methods and Protocols seeks to familiarize the researcher with the myriad of E. coli expression strains available and move E. coli closer to that ideal of the perfect host.

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Heterologous Gene Expression in E.coli

Methods and Protocols

Információk a könyvről

Teljes leírás

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