Human Retrovirus Protocols

Name: Human Retrovirus Protocols
Brand: Humana Press Inc.
SKU: 9781588294951
Price: 61266 HUF
Availability: InStock

Virology and Molecular Biology

Szerző: Tuofu Zhu

Nyelv:

Kötés: Kemény kötésű

Kiadó: Humana Press Inc.

Elérhetőség: Beszállítói készleten alacsony példányszámban

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Advances in our understanding of human retroviruses have been occurring at a very rapid pace and hav...

Információk a könyvről

Szerző

Tuofu Zhu

Nyelv

Angol

Kötés

Könyv - Kemény kötésű

Kiadva

2005

oldal

494

EAN

9781588294951

ISBN

1588294951

Enbook ID

01430457

Kiadó

Humana Press Inc.

Súly

1960

Méretek

155 x 235 x 33

Teljes leírás

Advances in our understanding of human retroviruses have been occurring at a very rapid pace and have contributed significantly to the development of new biotechnologies. In Human Retrovirus Protocols: Virology and Molecular Biology, recognized international researchers describe in detail the basic and state-of-the-art methods they have optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from proven methods for the isolation and detection of human retroviruses to advanced methods for exploring the interplay between the viruses and the host. Here, the researcher will find up-to-date techniques for the isolation and propagation of HIV, HTLV, and foamy virus from peripheral blood mononuclear cells (PBMC), blood monocytes, brain tissues, cerebrospinal fluids, semen, the vagina, and lymph nodes. There are also assays for determining the cell tropism of HIV-1, the coreceptor usage of HIV-1, and human gene expression with HIV-1 infection by microarrays, as well as for phenotyping HIV-1 infected monocytes and examining their fitness. Highlights include the detection and quantification of HIV-1 in resting CD4+, a new cloning system for making recombinant virus, cDNA microarrays, and the determination of genetic polymorphisms in two recently identified HIV-1 co-factors that are critical for HIV-1 infection. The protocols follow the highly praised Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. §State-of-the-art and highly practical, Human Retrovirus Protocols: Virology and Molecular Biology offers novice and experienced researchers alike proven tools essential for productive study and understanding of the human retroviruses.

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Human Retrovirus Protocols

Virology and Molecular Biology

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Teljes leírás

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