Könyv New Algorithms for Single Particle Cryo Electron Microscopic Image Processing Jan-Martin Kirves

New Algorithms for Single Particle Cryo Electron Microscopic Image Processing

Nyelv: Angol
Kötés: Puha kötésű
Elérhetőség: Beszállítói készleten
Küldés 8-11 napon belül
12 383 Ft
In this thesis four approaches to increase the quality and assure the validity of three-dimensional...

Információk a könyvről

Nyelv
Angol
Kötés
Könyv - Puha kötésű
Kiadva
2015
oldal
174
EAN
9783954049073
ISBN
3954049074
Enbook ID
12828738
Súly
234
Méretek
148 x 210 x 9

Teljes leírás

In this thesis four approaches to increase the quality and assure the validity of three-dimensional (3D) models generated within the single particle cryo-electron microscopy (CryoEM) workflow are introduced. ProteoPlex is a high-throughput technique and software tool for the evaluation of the most stabilizing biochemical conditions of macromolecular complexes. The validation technique 3D correlation of tilted pairs (3DCTP) can be applied alongside the single particle refinement cycle to detect model bias utilizing tilted pair images. A new analysis method identifies the presence of overfitting in high resolution CryoEM 3D structures. Finally, a set of criteria covering different model quality aspects of the generated 3D structure is introduced.

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