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Könyv Oligonucleotide Labeling and Detection by Serrs Ljiljana Fruk

Oligonucleotide Labeling and Detection by Serrs

Szerző: Ljiljana Fruk
Nyelv: Angol
Kötés: Puha kötésű
Kiadó: Scholars' Press
Elérhetőség: Kiadói készleten rendelésre
Küldés 17-27 napon belül
31 620 Ft
A number of methods for detecting specific DNA sequences have been used to provide data for diagnosi...

Információk a könyvről

Szerző
Ljiljana Fruk
Nyelv
Angol
Kötés
Könyv - Puha kötésű
Kiadva
2013
oldal
220
EAN
9783639512724
ISBN
3639512723
Enbook ID
06938560
Kiadó
Scholars' Press
Súly
327
Méretek
152 x 229 x 13

Teljes leírás

A number of methods for detecting specific DNA sequences have been used to provide data for diagnosis of diseases and examination of gene expression. The most favoured is fluorescence detection although the addition of fluorescent labels can be disadvantageous due to the complex and expensive labelling chemistry. In this work, a novel method of oligonucleotide labelling was devised and surface enhanced resonance Raman scattering (SERRS) used as a detection technique. A number of SERRS active dyes containing benzotriazole groups were prepared and attached to DNA using Diels Alder chemistry. Benzotriazole maleimide dye and fluorescein were used in the design of a novel class of biosensor named SERRS Beacons, in which both fluorescence and SERRS are utilized for detection of minute amounts of DNA in solution. Low detection limits, simplicity of both the labelling chemistry and SERRS surface preparation, makes SERRS an excellent technique for oligonucleotide detection with the possibility of use in the rapidly growing DNA microarray and proteomic area.

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