Pediatric Hematology

Name: Pediatric Hematology
Brand: Humana Press Inc.
SKU: 9781588290434
Price: 44920 HUF
Availability: InStock

Methods and Protocols

Szerző: Nicholas J. Goulden, Colin G. Steward

Nyelv:

Kötés: Kemény kötésű

Kiadó: Humana Press Inc.

Elérhetőség: Beszállítói készleten

Küldés 9-13 napon belül

44 920 Ft

Molecular techniques are now an integral part of the practice of modern pediatric hematology. In Ped...

Információk a könyvről

Szerző

Nicholas J. Goulden, Colin G. Steward

Nyelv

Angol

Kötés

Könyv - Kemény kötésű

Kiadva

2003

oldal

272

EAN

9781588290434

ISBN

1588290433

Enbook ID

01430203

Kiadó

Humana Press Inc.

Súly

1280

Méretek

155 x 235 x 24

Teljes leírás

Molecular techniques are now an integral part of the practice of modern pediatric hematology. In Pediatric Hematology: Methods and Protocols, hands-on masters of laboratory techniques describe their best methods for investigating and detecting a wide variety of hematological disorders. Here, the reader will find cutting-edge molecular protocols for the diagnosis of Fanconi anemia, dyskeratosis congenita, immunodeficiency, and most forms of hemoglobinopathy. In addition, there are detailed methods for molecular human platelet antigen genotyping, an effective PCR procedure for thrombophilia screening, and protocols for fluorescent in situ hybridization for cases in which insufficient metaphases are recovered or cryptic translocations are not visible. Since the measurement of minimal residual disease (MRD) provides a much more accurate risk-directed therapy, three methods are presented for detecting residual leukemia below the threshold of light microscopy, along with relatively simple, rapid, and inexpensive methods for the detection of MRD in ALL and AML. On the cutting-edge of technology, microarrays are used for the analysis of gene expression in childhood leukemia, and novel methods of graft engineering and reduced intensity conditioning regimens are applied to tissue typing for the assessment of donor/recipient chimerism. Each readily reproducible protocol is described in step-by-step detail and includes an introduction to the principle behind the method, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls.§Cutting-edge and highly practical, Pediatric Hematology: Methods and Protocols offers experimental and clinical investigators a gold-standard collection of reliable molecular techniques essential to the diagnosis, classification, and treatment of childhood hematological disorders.

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Pediatric Hematology

Methods and Protocols

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