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Könyv Plant Transposable Elements O. Nelson

Plant Transposable Elements

Szerző: O. Nelson
Nyelv: Angol
Kötés: Puha kötésű
Kiadó: Springer-Verlag New York Inc.
Elérhetőség: Beszállítói készleten alacsony példányszámban
Küldés 13-18 napon belül
40 448 Ft
Transposon tagging can work. Even though most of our understand ing about the factors that contribut...

Információk a könyvről

Szerző
O. Nelson
Nyelv
Angol
Kötés
Könyv - Puha kötésű
Kiadva
2012
oldal
404
EAN
9781468455526
ISBN
1468455524
Enbook ID
06797602
Kiadó
Springer-Verlag New York Inc.
Súly
802
Méretek
178 x 254 x 23

Teljes leírás

Transposon tagging can work. Even though most of our understand ing about the factors that contribute to a successful tagging experiment has been accumulated from a limited number of experiments using different transposable elements in different genetic backgrounds, it is still possible to draw some conclusions regarding the best experimental strategies for gene tagging. In our experience, Spm has proved to be a good element for transposon tagging. The frequency of recovering mutable alleles in duced by Spm is not significantly different from that for Ac-Ds or for Mu 6 (summarized in Ref. 22) and varies from about 10- to 10=zr:-8pm has the unique advantage, however, in that all of the members of thiSfumily that have been examined thus far are homologous to each other at the DNA level. Therefore, by combining molecular analysis with genetic segre gation, it is possible to identify and isolate alleles that are due to insertions of either autonomous or nonautonomous Spm elements. There are definite steps one can take to increase the chances of de tecting a transposition into the gene of interest. The most important step is to select a genetic background in which the desired phenotype will be easy to screen. If the phenotype is not likely to be mutable, then tester lines should be constructed so as to contain flanking markers that can aid in subsequent segregation analyses.

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