Könyv Screening for Reverse Cholesterol Transport efficiency bhaswati sengupta

Screening for Reverse Cholesterol Transport efficiency

Nyelv: Angol
Kötés: Puha kötésű
Kiadó: Scholars' Press
Elérhetőség: Beszállítói készleten
Küldés 8-11 napon belül
19 952 Ft
Generation of foam cells, an essential step for reverse cholesterol transport (RCT) studies, uses th...

Információk a könyvről

Nyelv
Angol
Kötés
Könyv - Puha kötésű
Kiadva
2014
oldal
96
EAN
9783639664034
ISBN
3639664035
Enbook ID
07175453
Súly
150
Méretek
152 x 229 x 6

Teljes leírás

Generation of foam cells, an essential step for reverse cholesterol transport (RCT) studies, uses the technique of receptor dependent macrophage loading with radiolabeled acetylated Low Density Lipoprotein (Ac-LDL). In this study, we used the ability of a biologically relevant detergent molecule, Lysophosphatidylcholine (Lyso PtdCho), to form mixed micelles with cholesterol or cholesteryl ester (CE) to generate macrophage foam cells. Fluorescent or radiolabelled cholesterol / Lyso PtdCho mixed micelles were prepared and incubated with RAW 264.7 or mouse peritoneal macrophages. Results showed that such micelles were quite stable at 4°C and retained the solubilized cholesterol during one month storage. Macrophages incubated with cholesterol or CE (unlabeled, fluorescently labeled or radiolabeled) / Lyso PtdCho mixed micelles accumulated CE as documented by microscopy, lipid staining, labeled oleate incorporation, and by thin layer chromatography (TLC). Such foam cells unloaded cholesterol when incubated with high density lipoprotein (HDL) and not with oxidized HDL (Ox-HDL). We propose that stable cholesterol or CE / Lyso PtdCho micelles would offer advantages over existing methods. O

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