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Könyv The Ubiquitin Code Manuel Rodriguez

The Ubiquitin Code

Szerző: Manuel Rodriguez, Rosa Barrio
Nyelv: Angol
Kötés: Kemény kötésű
Kiadó: Springer, Berlin
Elérhetőség: Beszállítói készleten
Küldés 10-13 napon belül
67 826 Ft
This volume details novel and popular approaches to decipher the Ubiquitin Code. Chapters guide read...

Információk a könyvről

Szerző
Manuel Rodriguez, Rosa Barrio
Nyelv
Angol
Kötés
Könyv - Kemény kötésű
Kiadva
2022
oldal
245
EAN
9781071628584
Enbook ID
41391834
Kiadó
Springer, Berlin
Súly
666
Méretek
178 x 254 x 20

Teljes leírás

This volume details novel and popular approaches to decipher the Ubiquitin Code. Chapters guide readers through ubiquitin chain complexity, methods to generate non-hydrolysable ubiquitin-NEDD8, a fully synthetic method for the linear synthesis of fluorescently labelled rhodamine LC3A and LC3B, a HECT ligase-mediated ubiquitylation in vitro, a RNAi-based screening to identify DUBs, a Yuh1 activity assay, a pull-down to isolate K48 and K63 chains using specific nanobodies, identification of NeDDylated protein based in a NEDD8R74K mutant, in vivo identification of NEDDylated proteins from liver diseases, UbL-ID method to identify ubiquitin-like modified proteins, a LC3/GABARAP capturing system, and methods to isolate peptides that are closely associated with proteasomes or trapped inside the core particle of proteasomes in eukaryotic cells, among others. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Ubiquitin Code aims to to be a useful practical guide to researches to help further their study in this field.

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Present Praise

MR Hillary Turyagyenda
7 209 Ft